Document 3308
 DOCN  M94A3308
 TI    Analysis of pol gene of a non-infectious HIV-1 clone.
 DT    9412
 AU    Nakano T; Sano K; Goto T; Morimatsu S; Ueda S; Ikuta K; Kato S; Nakai M;
       Department of Microbiology, Osaka Medical College, Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):106 (abstract no. PA0043). Unique
       Identifier : AIDSLINE ICA10/94369267
 AB    OBJECTIVE: We analyzed the nucleotide sequence of pol gene encoding
       protease (PR) in a non-infectious HIV-1 producing cell clone L-2, and
       examined the expression of pol gene products. METHODS: Conventional
       dideoxy-termination method was used to sequence PR coding region of L-2
       and Molt-4/LAV-1 cells. The expressions of PR, reverse transcriptase
       (RT), and integrase (IN) in these cells were investigated with IF test
       using specific antibodies. RESULTS: There was insertion mutation at the
       42nd base of PR coding region of L-2. A stop codon was made at the
       position of the 30th codon of PR. The expressions of PR, RT, and IN in
       L-2 cell were not detectable. DISCUSSIONS AND CONCLUSIONS: Since L-2
       lacked PR, it was considered that the intact gag polyprotein in released
       particles was not cleaved. We found a pol-unexpressing wild mutant of
       cultured cells, and the mutant may be useful to analyze further
       mechanism of the onset of AIDS in vivo.
 DE    Acquired Immunodeficiency Syndrome/ETIOLOGY  Cell Line  Cloning,
       Molecular  DNA Nucleotidyltransferases/GENETICS  Gene Expression  Gene
       Products, gag/GENETICS  *Genes, pol  Human
       HIV-1/*GENETICS/PATHOGENICITY  Mutation  Reverse Transcriptase/GENETICS
       Virulence/GENETICS  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).